Regulation of gene expression

Projet en recherche de financement

Project leader USTV (PROTEE)
Industrials partners AQUALOG, HYDRALOUP
Budget 1 249 K€
Year of labeling 2008

A growing number of anthropogenic xenobiotic pollutants regularly affect the aquatic environment. Some of these compounds are suspected or known to have effects on the endocrine system of marine organisms. These molecules are known as endocrine disruptors and have multiple modes of action: binding to hormonal receptors, modifying intrinsic concentrations of natural hormones acting on their production, their storage, transport, metabolism and excretion. Whatever their mode of action, their effects are deleterious to fish: dysfunctional thyroid function, behavioural disturbance, incomplete development of genitalia, decreased fertility, development of imposex ... the presence of endocrine disruptors in the environment, even in trace amounts, has a direct impact on the health of marine organisms (e.g. feminization of certain fish populations and vice versa) and, ultimately, perhaps on man. In order to complement existing knowledge on the modes of action and effects of endocrine disruptors, this project will study the impact of six different compounds with endocrine activity (E2, 11KT, Tamoxifen, Cyproterone acetate, BaP and 4NP) in the Mediterranean sea bass (Dicentrarchus labrax L.). In this instance, this approach requires molecular sequencing of the transcriptome of D. labrax and the manufacture a chip-specific to the cDNA of sea bass. This cDNA chip will be the basic tool to assess their impact on the health of wild sea bass in the Mediterranean. This data (groups of biomarkers) will be directly extrapolated to other Mediterranean fish.